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| Registros recuperados: 81 | |
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| Castilho,Camila S.; Marins-Sá,Luiz G.; Benedet,Rodrigo C.; Freitas,Thales R.O.. |
| The Brazilian Atlantic Rain Forest, one of the most endangered ecosystems worldwide, is also among the most important hotspots as regards biodiversity. Through intensive logging, the initial area has been reduced to around 12% of its original size. In this study we investigated the genetic variability and structure of the mountain lion, Puma concolor. Using 18 microsatellite loci we analyzed evidence of allele dropout, null alleles and stuttering, calculated the number of allele/locus, PIC, observed and expected heterozygosity, linkage disequilibrium, Hardy-Weinberg equilibrium, F IS, effective population size and genetic structure (MICROCHECKER, CERVUS, GENEPOP, FSTAT, ARLEQUIN, ONESAMP, LDNe, PCAGEN, GENECLASS software),we also determine whether there... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Araucaria Forest; Atlantic Rain Forest; Conservation; Genetic diversity; Microsatellite. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100009 |
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| de la Barra,Rodrigo; Uribe,Héctor; Latorre,Etel; San Primitivo,Fermín; Arranz,Juan. |
| Chilean sheep breeds have a significant founder effect since they descend from very few parents, in some cases population inbreeding has increased and in others there have been significant differentiation processes as compared to their original population. The objective of this study was to estimate the current status of genetic diversity in sheep herds, which comprise the main sheep breeds in Chile, using molecular methods. Animals from four breeding herds were sampled and analyzed. The breeds studied were Corriedale, Suffolk Down, Romney Marsh and Chilota, these are the predominant numerical pure breeds in Chile. From each breed population 40 animals were sampled. Genetic characterization was done using nine microsatellite molecular markers (SSR)... |
| Tipo: Journal article |
Palavras-chave: Microsatellite; Diversity; Sheep; Chile. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000400016 |
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| Muths, Delphine; Tessier, Emmanuel; Bourjea, Jerome. |
| The reef fauna connectivity of the West Indian Ocean (WIO) is one of the least studied globally. Here we use genetic analyses of the grouper Epinephelus merra (Bloch 1793) to determine patterns of connectivity and to identify barriers to dispersal in this WIO marine area. Phylogeographic and population-level analyses were conducted on cytochrome b sequences and microsatellites (13 loci) from 557 individuals sampled in 15 localities distributed across the West Indian Ocean. Additional samples from the Pacific Ocean were used to benchmark the WIO population structure. The high level of divergence revealed between Indian and Pacific localities (of about 4.5% in sequences) might be the signature of the major tectonic and climatic changes operating at the... |
| Tipo: Text |
Palavras-chave: Cytochrome b; Marine connectivity; Microsatellite; Reef fish; West Indian Ocean. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00189/30046/28566.pdf |
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| Abreu,Marcia Matos de; Pereira,Luiz Henrique Garcia; Vila,Vilma Barretto; Foresti,Fausto; Oliveira,Claudio. |
| Catfishes of the genus Pseudoplatystoma are very important species due to both their high commercial value and their ecological role as voracious predators. They undertake lengthy migratory movements during their life-cycle, this including reproductive migration which occurs from October to December in the rainy season. In the present study, seven microsatellite loci were analyzed to access genetic variability in two samples of P. reticulatum from the Upper Paraguay Basin. The loci were highly polymorphic (mean = 7.28). According to all analysis, the two samples of P. reticulatum revealed pronounced genetic differentiation. Fst value was 0.2290, Rst value 0.1067 and AMOVA 22.90% (Fst) and 10.67% (Rst), all being highly significant (p < 0.001). The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish; Population genetics; Microsatellite; Homing; Population structure. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400032 |
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| Lima,Ana Patrícia Souza de; Silva,Suzianny Maria Bezerra Cabral da; Oliveira,Karine Kelly Cavalcanti; Maggioni,Rodrigo; Coimbra,Maria Raquel Moura. |
| The shrimp industry has grown significantly over the past 10 years in Brazil, especially the farmed production of the exotic Pacific white shrimp, Litopenaeus vannamei. In 2004, this industry was marked by a productivity crisis, which stirred interest towards genetic improvement of shrimp stocks. Shrimp breeders importation was banned in Brazil by a govern Normative Instruction in 1997, as a sanitary precaution. Since then, broodstock replacement in hatcheries has been based on domestic stocks, raising concerns on the decline of genetic diversity and if the existing diversity would allow effective genetic improvement programs. In the present research, genetic parameters such as number of alleles, effective allele number, expected and observed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Litopenaeus vannamei; Population genetics; Genetic diversity; Microsatellite. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000200011 |
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| Possamai,Mari Helen Pagani; Battilana,Jaqueline; Paludo,Ediane; Herkenhoff,Marcos Edgar; Pértile,Fábio; Lima-Rosa,Carlos André da Veiga. |
| This study aimed to investigate the genetic variability of two Brazilian free range (Caipira) chickens lines using microsatellites analysis of ten loci. It was collected a total of 99 blood samples, which 49 were from Paraíso Pedrês (PP) and 50 were from Rubro Negra (RN) lines. The amplification of the DNA fragments was performed by polymerase chain reaction (PCR) and the genotyping was conduct using ABI 3130 sequencer. The allele number variation was among 3 (LEI0254) to 32 (LEI0212) in the PP line, and 4 (LEI0254) to 31 (LEI0212) in the RN line. The allelic average per locus was 13.3 and 13.1 in the PP and RN lines, respectively. The average observed and the expected heterozygosity were 0.650 and 0.820 in the PP line, and 0.671 and 0.804 in the RN line.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: (Caipira) broiler line; (Caipira) layer line; Microsatellite; Polymorphism. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500877 |
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| Mignotte, Antoine; Garros, Claire; Dellicour, Simon; Jacquot, Maude; Gilbert, Marius; Gardès, Laetitia; Balenghien, Thomas; Duhayon, Maxime; Rakotoarivony, Ignace; De Wavrechin, Maïa; Huber, Karine. |
| Background In the last two decades, recurrent epizootics of bluetongue virus and Schmallenberg virus have been reported in the western Palearctic region. These viruses affect domestic cattle, sheep, goats and wild ruminants and are transmitted by native hematophagous midges of the genus Culicoides (Diptera: Ceratopogonidae). Culicoides dispersal is known to be stratified, i.e. due to a combination of dispersal processes occurring actively at short distances and passively or semi-actively at long distances, allowing individuals to jump hundreds of kilometers. Methods Here, we aim to identify the environmental factors that promote or limit gene flow of Culicoides obsoletus, an abundant and widespread vector species in Europe, using an innovative framework... |
| Tipo: Text |
Palavras-chave: Culicoides obsoletus; Landscape genetics; Microsatellite; Dispersal; Palearctic region. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00678/79022/81428.pdf |
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| Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie; Boudry, Pierre. |
| Crassostrea angulata and Crassostrea gigas, two cupped oysters of Asian origin (O'Foighil et al, 1998), have been introduced successively in Europe during the XVlth and the XXth century (Grizel et Héral, 1991). The present populations of C. angulata appear to be limited to the south of Spain and Portugal, whereas the distribution of C. gigas in Europe is rising because of the development of oyster farming. The distinction between the two subspecies in based on a RFLP marker of the mitochondrial genome (COI) which shows a strong genetic differentiation between the two subspecies populations (Boudry et al., 1998). On the contrary, the nuclear markers (microsatellites) showed a smaller genetic differentiation between them (Huvet et al, under press). The... |
| Tipo: Text |
Palavras-chave: Microsatellite; Hybridation; Crassostrea angulata; Crassostrea gigas; Huîtres; Génétique. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3282.pdf |
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| Han,; Kim, Ji Hyung; Renault, Tristan; Choresca, Casiano, Jr.; Shin, Sang Phil; Jun, Jin Woo; Park, Se Chang. |
| Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA,... |
| Tipo: Text |
Palavras-chave: Cyprinid herpes virus 3; Glycoprotein gene; Koi herpesvirus; Microsatellite; Polymorphism; Phylogenetic tree. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00118/22940/20768.pdf |
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| Rabello,Edenilson; Souza,Adriane Nunes de; Saito,Daniel; Tsai,Siu Mui. |
| This study assessed the abundance of microsatellites, or simple sequence repeats (SSR), in 19 Eucalyptus EST libraries from FORESTs, containing cDNA sequences from five species: E. grandis, E. globulus, E. saligna, E. urophylla and E. camaldulensis. Overall, a total of 11,534 SSRs and 8,447 SSR-containing sequences (25.5% of total ESTs) were identified, with an average of 1 SSR/2.5 kb when considering all motifs and 1 SSR/3.1 kb when mononucleotides were not included. Dimeric repeats were the most abundant (41.03%), followed by trimerics (36.11%) and monomerics (19.59%). The most frequent motifs were A/T (87.24%) for monomerics, AG/CT (94.44%) for dimerics, CCG/CGG (37.87%) for trimerics, AAGG/CCTT (18.75%) for tetramerics, AGAGG/CCTCT (14.04%) for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eucalyptus; EST; Microsatellite; Simple sequence repeat (SSR); Molecular marker. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400013 |
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| Oliveira-Klein,Mariana de; Cardoso-dos-Santos,Augusto César; Tagliani-Ribeiro,Alice; Fagundes,Nelson Rosa; Matte,Ursula; Schuler-Faccini,Lavinia. |
| Abstract Insulin-like growth factor (IGF-1) is an important peptide hormone involved in the reproduction and fetal development of mammals, and it is suggested that it may influence the human twinning rate. This study aimed to test such possible association, investigating the genetic polymorphisms IGF1 (CA)n and IGFBP3 rs2854744 in the population from Candido Godoi (CG), a small city located in the South of Brazil that has a high prevalence of twin births. A case-control study was performed comprising a total of 39 cases (representing about 40% of the mothers of twins who were born in CG after 1995) and 214 controls (mothers of non-twin children), 97 of whom were living in CG while 117 were living in Porto Alegre. DNA was extracted from blood leucocytes and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insulin-like growth factor; Founder effect; Microsatellite; Reproduction; Twinning. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500775 |
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| Gaino,Ana Paula Silva Campos; Moraes,Mario Luiz Teixeira de; Moreira,Juliana Prado; Cardin,Laila Toniol; Moraes,Marcela Aparecida; Silva,Alexandre Marques da; Freitas,Miguel Luiz Menezes; Sebbenn,Alexandre Magno. |
| The aims of this study were to investigate the mating system of a fragmented population of the dioecious tropical tree Myracrodruon urundeuva Allemão, using five microsatellite loci and the mixed mating and correlated mating models. The study was conducted in the Estação Ecológica de Paulo de Farias (436 ha), where the population occupies about 142 ha. The mating system was estimated using 514 open-pollinated offspring, collected from 30 seed-trees. Estimates of the multilocus outcrossing rate confirm that the species is dioecious (t m = 1.0). Low levels of mating among relatives were detected in the population (1 - t s = 0.020). The estimate of paternity correlation (r p(m)) indicated that offsprings were composed of mixtures of half-sibs and full-sibs,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Correlated matings; Fragmentation; Microsatellite; Variance effective population size. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042011000400008 |
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| Registros recuperados: 81 | |
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